Journal: Cellular immunology

Article Title: In vitro ovarian tumor-conditioned CD163+ human macrophages retain phagocytic response to CD47 blockade.

doi: 10.1016/j.cellimm.2025.104932

Figure Lengend Snippet: Fig. 2. Tumor conditioning induces macrophage CD163 and IL-10 gene expression. Tumor-conditioned human monocyte-derived macrophages (M(A2780)) were generated and their gene expression was compared to control macrophages by RT-qPCR. A-F: Presents gene expression of polarization markers (CD163, IL-10, and TNF-α) and phagocytosis checkpoints (SIRPα, LILRB1, and Siglec-10) in tumor-associated macrophages compared to controls. Statistics: comparisons were made against controls using paired t-tests and a significance level of p = 0.05. When indicated by a logarithmic y-axis, comparisons were made on log-transformed data. Data are presented as individual data points with mean ± SD. N = 4.

Article Snippet: The plates were read at 450/ 620 nm and a standard curve ranging from 0.625 to 8 μg/L was prepared using recombinant human LILRB1 (R&D systems, 8989-T2). sCD206 (samples diluted 1:5) [34], and sSIRPα (samples diluted 1:10) [35] were measured as previously reported in detail.

Techniques: Gene Expression, Derivative Assay, Generated, Control, Quantitative RT-PCR, Transformation Assay

Journal: Cellular immunology

Article Title: In vitro ovarian tumor-conditioned CD163+ human macrophages retain phagocytic response to CD47 blockade.

doi: 10.1016/j.cellimm.2025.104932

Figure Lengend Snippet: Fig. 3. Tumor cell conditioning induces CD163, CD206, CD80 and LILRB1 on the membrane protein level. Tumor-conditioned human monocyte-derived macrophages (M(A2780)) were generated and their membrane protein expression compared to control macrophages by flow cytometry. The gating strategy is presented in Supplementary fig. 1. A-G: Presents median fluorescence intensities (MFI) of polarization markers (CD163, CD206, and CD80) and MFI values and percent macrophages positive for phagocytosis checkpoints (LILRB1, and Siglec-10). Statistics: comparisons were made against controls using paired t-tests and a significance level of p = 0.05. When indicated by a logarithmic y-axis, comparisons were made on log-transformed data. Data are presented as individual data points with mean ± SD. N = 9.

Article Snippet: The plates were read at 450/ 620 nm and a standard curve ranging from 0.625 to 8 μg/L was prepared using recombinant human LILRB1 (R&D systems, 8989-T2). sCD206 (samples diluted 1:5) [34], and sSIRPα (samples diluted 1:10) [35] were measured as previously reported in detail.

Techniques: Membrane, Derivative Assay, Generated, Expressing, Control, Flow Cytometry, Fluorescence, Transformation Assay

Journal: NPJ Vaccines

Article Title: Computationally designed Spike antigens induce neutralising responses against the breadth of SARS-COV-2 variants

doi: 10.1038/s41541-024-00950-9

Figure Lengend Snippet: Surface representation of the extra-virion region of the Spike protein of SARS-CoV-2. The three subunits are coloured in pale yellow, pale blue, and grey. The structural domains – N-terminal domain (NTD), receptor binding domain (RBD), C-terminal domain of the S1 region (S1-CTD) and the stalk region (S2) are highlighted by green, black, magenta, and yellow-brown outlines respectively. The mutations reported in different variants are coloured as red sphere in the surface representation and indicated by red lines in the linear representation. The mutations introduced in the spike vaccine antigens are coloured as orange spheres in the surface representation and indicated by orange lines in the linear representation for T2_32, T2_35, T2_36 and T2_32_mFur. The surface representation was generated and rendered using PyMol using PDB id. 7ZR9 .

Article Snippet: Correct expression of T2_32 was confirmed by Western blot (40150-T62, Sino Biological; China) with cell lysates from HEK293 cells harvested 24 h after infection (MOI 2) with MVA T2_32.

Techniques: Binding Assay, Generated